A detailed understanding of human-microbe symbiosis requires a precise characterization of human-associated microorganisms, the human microbiome. To progress towards this ambitious goal it is of utmost importance that the data generated in each of many large projects involved in human metagenome research be optimally comparable. The International Human Microbiome Standards (IHMS) project coordinated the development of standard operating procedures designed to optimize data quality and comparability in the human microbiome field.

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IHMS consist in differents work package :

SOPS

SOPs
for sample collection,
identification and extraction

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SOPs
for sequencing
 
 

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SOPs
for data analysis
 
 

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Standards and recommandations for metagenomics long continuous reference

IHMS SOP 008

Standards and recommandations for metagenomics short reads
 

IHMS SOP 009

Standards and recommandations for metagenomics short reads
 

IHMS SOP 010

Assessment of analysis for visualisation options

IHMS SOP 011

Standards for overall quality analysis
 

IHMS SOP 012

Standards for phylogenetic assignments
 

IHMS SOP 013

Orthologous groups for function assigment
 

IHMS SOP 014

TEAM

The 8 partners of IHMS and 15 contributors across 12 different countries further participated in a study designed to identify the critical features and optimal protocols for DNA preparation. Contributors were offered to treat aliquots of two fecal samples using their own lab procedures.

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Publications

  • BMC Microbiology - 2012
  • BMC Microbiology - 2014

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